Primer Details for TRINITY_DN38037_c0_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCAAACCGAACAAAGAAGGG | ATGAATAAAAACCATTGACACCGT | Start: 133 |
| Tm | 59.314 | 57.66 | End: 232 |
| Size | 22 | 24 | 100 |
| Primer 2 details |
|---|
| Sequence | ATGTCAAACCGAACAAAGAAGGG | TGAATAAAAACCATTGACACCGT | Start: 132 |
| Tm | 59.682 | 57.224 | End: 231 |
| Size | 23 | 23 | 100 |
| Primer 3 details |
|---|
| Sequence | TCCTCTTGTATTATGAGCTTTTGTCA | CGTACACCCTTCTTTGTTCGG | Start: 16 |
| Tm | 58.606 | 59.204 | End: 160 |
| Size | 26 | 21 | 145 |
| Primer 4 details |
|---|
| Sequence | ATGTCAAACCGAACAAAGAAGGG | TGAATAAAAACCATTGACACCGTTG | Start: 132 |
| Tm | 59.682 | 59.02 | End: 231 |
| Size | 23 | 25 | 100 |
| Primer 5 details |
|---|
| Sequence | TGTCAAACCGAACAAAGAAGGG | ATGAATAAAAACCATTGACACCGTTG | Start: 133 |
| Tm | 59.314 | 59.355 | End: 232 |
| Size | 22 | 26 | 100 |
