Primer Details for TRINITY_DN38031_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTTCAACTACCCTTCCCACT | TTTTTCCTCTTTTGTTGCATTCTTT | Start: 10 |
| Tm | 57.258 | 57.08 | End: 114 |
| Size | 22 | 25 | 105 |
| Primer 2 details |
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| Sequence | AAATTTCAACTACCCTTCCCACT | TTTTTCCTCTTTTGTTGCATTCTTT | Start: 9 |
| Tm | 57.925 | 57.08 | End: 114 |
| Size | 23 | 25 | 106 |
| Primer 3 details |
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| Sequence | TCAACTACCCTTCCCACTATTTCT | TTTTTCCTCTTTTGTTGCATTCTTT | Start: 14 |
| Tm | 58.901 | 57.08 | End: 114 |
| Size | 24 | 25 | 101 |
| Primer 4 details |
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| Sequence | GGGCAAAGTAAATTTCAACTACCCT | TTTTTCCTCTTTTGTTGCATTCTTT | Start: 0 |
| Tm | 59.755 | 57.08 | End: 114 |
| Size | 25 | 25 | 115 |
| Primer 5 details |
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| Sequence | AATTTCAACTACCCTTCCCACT | TGCATTAATTGATGATCTTATGGGTT | Start: 10 |
| Tm | 57.258 | 57.519 | End: 165 |
| Size | 22 | 26 | 156 |
