Primer Details for TRINITY_DN37974_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTTATCCAAATTTAAACGACATTGT | GGTTTGCTCTTCCTTTTCATCGT | Start: 4 |
| Tm | 58.639 | 59.747 | End: 246 |
| Size | 27 | 23 | 243 |
| Primer 2 details |
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| Sequence | TGGTTATCCAAATTTAAACGACATTGT | AGGTTTGCTCTTCCTTTTCATCG | Start: 4 |
| Tm | 58.639 | 59.496 | End: 247 |
| Size | 27 | 23 | 244 |
| Primer 3 details |
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| Sequence | AACGACATTGTTTTTCAAGATCTTTT | GGTTTGCTCTTCCTTTTCATCGT | Start: 20 |
| Tm | 57.298 | 59.747 | End: 246 |
| Size | 26 | 23 | 227 |
| Primer 4 details |
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| Sequence | AAACGACATTGTTTTTCAAGATCTTT | GGTTTGCTCTTCCTTTTCATCGT | Start: 19 |
| Tm | 57.298 | 59.747 | End: 246 |
| Size | 26 | 23 | 228 |
| Primer 5 details |
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| Sequence | TGGTTATCCAAATTTAAACGACATTG | GGTTTGCTCTTCCTTTTCATCGT | Start: 4 |
| Tm | 57.25 | 59.747 | End: 246 |
| Size | 26 | 23 | 243 |
