Primer Details for TRINITY_DN37871_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGAAGAAAGTGATGGCAAAAAGA | CGGTTTGGCTTTCTGTTTTAAGC | Start: 127 |
| Tm | 59.639 | 59.511 | End: 226 |
| Size | 25 | 23 | 100 |
| Primer 2 details | |||
| Sequence | AGTGAAGAAAGTGATGGCAAAAAG | CGGTTTGGCTTTCTGTTTTAAGC | Start: 127 |
| Tm | 58.405 | 59.511 | End: 226 |
| Size | 24 | 23 | 100 |
| Primer 3 details | |||
| Sequence | AGTGAAGAAAGTGATGGCAAAAA | CGGTTTGGCTTTCTGTTTTAAGC | Start: 127 |
| Tm | 57.403 | 59.511 | End: 226 |
| Size | 23 | 23 | 100 |
| Primer 4 details | |||
| Sequence | AAGTGAAGAAAGTGATGGCAAAA | CGGTTTGGCTTTCTGTTTTAAGC | Start: 126 |
| Tm | 57.403 | 59.511 | End: 226 |
| Size | 23 | 23 | 101 |
| Primer 5 details | |||
| Sequence | AGTGAAGAAAGTGATGGCAAAAAGA | TTCGGTTTGGCTTTCTGTTTT | Start: 127 |
| Tm | 59.639 | 57.122 | End: 228 |
| Size | 25 | 21 | 102 |
