ssr_primer
Primer Details for TRINITY_DN37854_c0_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCGACACTTGGTAGCCT | TGGAGTCATTAACCCACGAGT | Start: 35 |
| Tm | 60.252 | 58.746 | End: 208 |
| Size | 20 | 21 | 174 |
| Primer 2 details |
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| Sequence | TTCTCCGACACTTGGTAGCC | TGGAGTCATTAACCCACGAGT | Start: 34 |
| Tm | 59.393 | 58.746 | End: 208 |
| Size | 20 | 21 | 175 |
| Primer 3 details |
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| Sequence | ACCCATCACTTCTCCGACAC | TGGAGTCATTAACCCACGAGT | Start: 25 |
| Tm | 59.391 | 58.746 | End: 208 |
| Size | 20 | 21 | 184 |
| Primer 4 details |
|---|
| Sequence | TCTCCGACACTTGGTAGCCT | TGGAGTCATTAACCCACGAGTT | Start: 35 |
| Tm | 60.252 | 59.364 | End: 208 |
| Size | 20 | 22 | 174 |
| Primer 5 details |
|---|
| Sequence | TCTCCGACACTTGGTAGCCT | ATGGAGTCATTAACCCACGAGT | Start: 35 |
| Tm | 60.252 | 59.162 | End: 209 |
| Size | 20 | 22 | 175 |
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