ssr_primer
Primer Details for TRINITY_DN3774_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCACCTCTCCCTAACCA | GTGGTTCAATAGGGGAGCGA | Start: 140 |
| Tm | 59.957 | 59.46 | End: 272 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
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| Sequence | CCTCCACCTCTCCCTAACCA | AGTGGTTCAATAGGGGAGCG | Start: 140 |
| Tm | 59.957 | 59.458 | End: 273 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | ACAAGCCTGCACCTACTGAA | TGGTTAGGGAGAGGTGGAGG | Start: 50 |
| Tm | 59.235 | 59.957 | End: 159 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
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| Sequence | AACAAGCCTGCACCTACTGA | TGGTTAGGGAGAGGTGGAGG | Start: 49 |
| Tm | 59.235 | 59.957 | End: 159 |
| Size | 20 | 20 | 111 |
| Primer 5 details |
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| Sequence | ACAAGCCTGCACCTACTGAA | TTGAGATGTTGGGTGTGCGA | Start: 50 |
| Tm | 59.235 | 59.892 | End: 214 |
| Size | 20 | 20 | 165 |
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