Primer Details for TRINITY_DN37502_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCTCAAGGTGTGTTGTACTCC | CGGTTCTTTTAGTGTGAGATCTTCG | Start: 31 |
| Tm | 59.93 | 59.939 | End: 153 |
| Size | 23 | 25 | 123 |
| Primer 2 details | |||
| Sequence | AGTCTCAAGGTGTGTTGTACTCC | AGTGTGAGATCTTCGACAAATGA | Start: 31 |
| Tm | 59.93 | 57.861 | End: 143 |
| Size | 23 | 23 | 113 |
| Primer 3 details | |||
| Sequence | GTCTCAAGGTGTGTTGTACTCC | CGGTTCTTTTAGTGTGAGATCTTCG | Start: 32 |
| Tm | 58.609 | 59.939 | End: 153 |
| Size | 22 | 25 | 122 |
| Primer 4 details | |||
| Sequence | GTCTCAAGGTGTGTTGTACTCC | AGTGTGAGATCTTCGACAAATGA | Start: 32 |
| Tm | 58.609 | 57.861 | End: 143 |
| Size | 22 | 23 | 112 |
| Primer 5 details | |||
| Sequence | AGTCTCAAGGTGTGTTGTACTCC | TCTTCGGTTCTTTTAGTGTGAGA | Start: 31 |
| Tm | 59.93 | 57.475 | End: 157 |
| Size | 23 | 23 | 127 |
