Primer Details for TRINITY_DN37285_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGATATATTACTGCCCGTTGC | TCTGCAATTGGTCATGGTCCT | Start: 0 |
| Tm | 57.336 | 59.645 | End: 104 |
| Size | 22 | 21 | 105 |
| Primer 2 details | |||
| Sequence | AGGGATGTAGGACCATGACCA | TGGAGGTTATTAGATTGCAAGCT | Start: 76 |
| Tm | 59.99 | 57.884 | End: 245 |
| Size | 21 | 23 | 170 |
| Primer 3 details | |||
| Sequence | AGGGATGTAGGACCATGACCA | GCAAGCTTAATTTCAACATCACTGC | Start: 76 |
| Tm | 59.99 | 60.164 | End: 229 |
| Size | 21 | 25 | 154 |
| Primer 4 details | |||
| Sequence | AGGACCATGACCAATTGCAGA | TGGAGGTTATTAGATTGCAAGCT | Start: 84 |
| Tm | 59.645 | 57.884 | End: 245 |
| Size | 21 | 23 | 162 |
| Primer 5 details | |||
| Sequence | GTGATATATTACTGCCCGTTGC | TCTGCAATTGGTCATGGTCC | Start: 0 |
| Tm | 57.336 | 58.162 | End: 104 |
| Size | 22 | 20 | 105 |
