Primer Details for TRINITY_DN3725_c0_g1_i1 | |||
|---|---|---|---|
| (A)14: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAGTTAGTAACCGATGTTCTTCT | AGGTGAACCAACAAGAAGAGCA | Start: 70 |
| Tm | 59.754 | 60.092 | End: 259 |
| Size | 25 | 22 | 190 |
| Primer 2 details | |||
| Sequence | ACCAGTTAGTAACCGATGTTCTTCT | GGTGAACCAACAAGAAGAGCA | Start: 70 |
| Tm | 59.754 | 58.702 | End: 258 |
| Size | 25 | 21 | 189 |
| Primer 3 details | |||
| Sequence | ACCAGTTAGTAACCGATGTTCTTC | AGGTGAACCAACAAGAAGAGCA | Start: 70 |
| Tm | 58.523 | 60.092 | End: 259 |
| Size | 24 | 22 | 190 |
| Primer 4 details | |||
| Sequence | ACCAGTTAGTAACCGATGTTCTT | AGGTGAACCAACAAGAAGAGCA | Start: 70 |
| Tm | 57.521 | 60.092 | End: 259 |
| Size | 23 | 22 | 190 |
| Primer 5 details | |||
| Sequence | ACCAGTTAGTAACCGATGTTCTTC | GGTGAACCAACAAGAAGAGCA | Start: 70 |
| Tm | 58.523 | 58.702 | End: 258 |
| Size | 24 | 21 | 189 |
