Primer Details for TRINITY_DN37235_c0_g1_i1 | |||
|---|---|---|---|
| (TA)6: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAAGGAATCAAATAACGCCAA | TGTTGTGTTCGTGTTAGTGGT | Start: 12 |
| Tm | 57.539 | 58.025 | End: 145 |
| Size | 23 | 21 | 134 |
| Primer 2 details | |||
| Sequence | AACAAAGGAATCAAATAACGCCA | TGTTGTGTTCGTGTTAGTGGT | Start: 11 |
| Tm | 57.539 | 58.025 | End: 145 |
| Size | 23 | 21 | 135 |
| Primer 3 details | |||
| Sequence | ACAAAGGAATCAAATAACGCCAAAC | TGTTGTGTTCGTGTTAGTGGT | Start: 12 |
| Tm | 59.306 | 58.025 | End: 145 |
| Size | 25 | 21 | 134 |
| Primer 4 details | |||
| Sequence | AAGGAATCAAATAACGCCAAACT | TGTTGTGTTCGTGTTAGTGGT | Start: 15 |
| Tm | 57.278 | 58.025 | End: 145 |
| Size | 23 | 21 | 131 |
| Primer 5 details | |||
| Sequence | ACAAAGGAATCAAATAACGCCAA | TGTTGTGTTCGTGTTAGTGGTT | Start: 12 |
| Tm | 57.539 | 58.664 | End: 145 |
| Size | 23 | 22 | 134 |
