Primer Details for TRINITY_DN37156_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACGCATACATACTTATTAAAAACCG | GAGGAGGGTGGCGCAAAA | Start: 173 |
| Tm | 58.882 | 60.282 | End: 321 |
| Size | 27 | 18 | 149 |
| Primer 2 details |
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| Sequence | ACACGCATACATACTTATTAAAAACCG | CGACTGGACACTTGTCGCG | Start: 173 |
| Tm | 58.882 | 61.365 | End: 346 |
| Size | 27 | 19 | 174 |
| Primer 3 details |
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| Sequence | CACGCATACATACTTATTAAAAACCGA | GAGGAGGGTGGCGCAAAA | Start: 174 |
| Tm | 58.67 | 60.282 | End: 321 |
| Size | 27 | 18 | 148 |
| Primer 4 details |
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| Sequence | CACGCATACATACTTATTAAAAACCGA | CGACTGGACACTTGTCGCG | Start: 174 |
| Tm | 58.67 | 61.365 | End: 346 |
| Size | 27 | 19 | 173 |
| Primer 5 details |
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| Sequence | ACGCATACATACTTATTAAAAACCGA | GAGGAGGGTGGCGCAAAA | Start: 175 |
| Tm | 57.577 | 60.282 | End: 321 |
| Size | 26 | 18 | 147 |
