Primer Details for TRINITY_DN37113_c0_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATGGGCATTGAGTTAAATCAGTGA | TGGAAGATAGCTCAAACGCCT | Start: 97 |
| Tm | 59.284 | 59.445 | End: 196 |
| Size | 25 | 21 | 100 |
| Primer 2 details | |||
| Sequence | ATGGGCATTGAGTTAAATCAGTGA | TTGGAAGATAGCTCAAACGCCT | Start: 98 |
| Tm | 58.736 | 60.027 | End: 197 |
| Size | 24 | 22 | 100 |
| Primer 3 details | |||
| Sequence | TGGGCATTGAGTTAAATCAGTGA | TTTGGAAGATAGCTCAAACGCC | Start: 99 |
| Tm | 58.339 | 59.253 | End: 198 |
| Size | 23 | 22 | 100 |
| Primer 4 details | |||
| Sequence | AATGGGCATTGAGTTAAATCAGTG | TGGAAGATAGCTCAAACGCCT | Start: 97 |
| Tm | 58.031 | 59.445 | End: 196 |
| Size | 24 | 21 | 100 |
| Primer 5 details | |||
| Sequence | ATGGGCATTGAGTTAAATCAGTG | TTGGAAGATAGCTCAAACGCCT | Start: 98 |
| Tm | 57.413 | 60.027 | End: 197 |
| Size | 23 | 22 | 100 |
