Primer Details for TRINITY_DN37095_c0_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACTTTTGTCCAAGGATCTACCA | TGAATGTGCACACTTGATTTGA | Start: 96 |
| Tm | 59.65 | 57.085 | End: 304 |
| Size | 24 | 22 | 209 |
| Primer 2 details | |||
| Sequence | TCACTTTTGTCCAAGGATCTACCA | AGTTGAATGTGCACACTTGATT | Start: 96 |
| Tm | 59.65 | 57.076 | End: 307 |
| Size | 24 | 22 | 212 |
| Primer 3 details | |||
| Sequence | TCACTTTTGTCCAAGGATCTACCA | AGGGCTTGAGTATTAAGAGTTGTGT | Start: 96 |
| Tm | 59.65 | 59.987 | End: 237 |
| Size | 24 | 25 | 142 |
| Primer 4 details | |||
| Sequence | TCACTTTTGTCCAAGGATCTACCA | GTTGAATGTGCACACTTGATTTGA | Start: 96 |
| Tm | 59.65 | 58.957 | End: 306 |
| Size | 24 | 24 | 211 |
| Primer 5 details | |||
| Sequence | TCACTTTTGTCCAAGGATCTACCA | AGTTGAATGTGCACACTTGATTTG | Start: 96 |
| Tm | 59.65 | 58.955 | End: 307 |
| Size | 24 | 24 | 212 |
