ssr_primer
Primer Details for TRINITY_DN36994_c0_g1_i1 |
|---|
| (CCT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCACTTTTGCCCCTCAACA | AGCGATCAGGGGTGTCTTTG | Start: 14 |
| Tm | 60.033 | 60.036 | End: 133 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
|---|
| Sequence | ACCACTTTTGCCCCTCAACA | CAGCGATCAGGGGTGTCTTT | Start: 14 |
| Tm | 60.033 | 60.036 | End: 134 |
| Size | 20 | 20 | 121 |
| Primer 3 details |
|---|
| Sequence | CTCCTCCCCTACAACCTCCA | AAACACAGCAACCGCCAATC | Start: 267 |
| Tm | 59.957 | 59.968 | End: 408 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | ACCACTTTTGCCCCTCAACA | TGGAGGTTGTAGGGGAGGAG | Start: 14 |
| Tm | 60.033 | 59.957 | End: 286 |
| Size | 20 | 20 | 273 |
| Primer 5 details |
|---|
| Sequence | AAAGACACCCCTGATCGCTG | TGGAGGTTGTAGGGGAGGAG | Start: 115 |
| Tm | 60.036 | 59.957 | End: 286 |
| Size | 20 | 20 | 172 |
(A)11ttaatgtagccaaatttgcaattttatg(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCACTTTTGCCCCTCAACA | AGCGATCAGGGGTGTCTTTG | Start: 14 |
| Tm | 60.033 | 60.036 | End: 133 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
|---|
| Sequence | ACCACTTTTGCCCCTCAACA | CAGCGATCAGGGGTGTCTTT | Start: 14 |
| Tm | 60.033 | 60.036 | End: 134 |
| Size | 20 | 20 | 121 |
| Primer 3 details |
|---|
| Sequence | CTCCTCCCCTACAACCTCCA | AAACACAGCAACCGCCAATC | Start: 267 |
| Tm | 59.957 | 59.968 | End: 408 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | ACCACTTTTGCCCCTCAACA | TGGAGGTTGTAGGGGAGGAG | Start: 14 |
| Tm | 60.033 | 59.957 | End: 286 |
| Size | 20 | 20 | 273 |
| Primer 5 details |
|---|
| Sequence | AAAGACACCCCTGATCGCTG | TGGAGGTTGTAGGGGAGGAG | Start: 115 |
| Tm | 60.036 | 59.957 | End: 286 |
| Size | 20 | 20 | 172 |
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