Primer Details for TRINITY_DN35830_c0_g1_i1 | |||
|---|---|---|---|
| (GT)6: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAACCTTTAGAACATGCACATT | CCCCCATTCTCACTTTCCCC | Start: 222 |
| Tm | 57.21 | 60.033 | End: 335 |
| Size | 23 | 20 | 114 |
| Primer 2 details | |||
| Sequence | AGAACATGCACATTAAAATGCTCA | CCCCCATTCTCACTTTCCCC | Start: 231 |
| Tm | 57.972 | 60.033 | End: 335 |
| Size | 24 | 20 | 105 |
| Primer 3 details | |||
| Sequence | AGATACAACCTTTAGAACATGCACA | CCCCCATTCTCACTTTCCCC | Start: 218 |
| Tm | 58.762 | 60.033 | End: 335 |
| Size | 25 | 20 | 118 |
| Primer 4 details | |||
| Sequence | AGAACATGCACATTAAAATGCTCAA | CCCCCATTCTCACTTTCCCC | Start: 231 |
| Tm | 58.543 | 60.033 | End: 335 |
| Size | 25 | 20 | 105 |
| Primer 5 details | |||
| Sequence | GATACAACCTTTAGAACATGCACA | CCCCCATTCTCACTTTCCCC | Start: 219 |
| Tm | 57.505 | 60.033 | End: 335 |
| Size | 24 | 20 | 117 |
