Primer Details for TRINITY_DN35730_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCGGTCCATAGAAATGCA | TGGAGTAGTGAGACATCATCTATGC | Start: 65 |
| Tm | 60.465 | 59.757 | End: 199 |
| Size | 20 | 25 | 135 |
| Primer 2 details |
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| Sequence | TGCCCGGTCCATAGAAATGC | TGGAGTAGTGAGACATCATCTATGC | Start: 64 |
| Tm | 60.465 | 59.757 | End: 199 |
| Size | 20 | 25 | 136 |
| Primer 3 details |
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| Sequence | GCCCGGTCCATAGAAATGCA | TGATGGAGTAGTGAGACATCATCT | Start: 65 |
| Tm | 60.465 | 58.547 | End: 202 |
| Size | 20 | 24 | 138 |
| Primer 4 details |
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| Sequence | TGCCCGGTCCATAGAAATGC | TGATGGAGTAGTGAGACATCATCT | Start: 64 |
| Tm | 60.465 | 58.547 | End: 202 |
| Size | 20 | 24 | 139 |
| Primer 5 details |
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| Sequence | GCCCGGTCCATAGAAATGCA | GGAGTAGTGAGACATCATCTATGCT | Start: 65 |
| Tm | 60.465 | 59.523 | End: 198 |
| Size | 20 | 25 | 134 |
