Primer Details for TRINITY_DN35543_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTGTACTGGCAAATTAATGTGA | CGACACGGAGCCACCTAAAA | Start: 120 |
| Tm | 58.679 | 60.32 | End: 258 |
| Size | 24 | 20 | 139 |
| Primer 2 details | |||
| Sequence | AGGTGTACTGGCAAATTAATGTGA | ACACGGAGCCACCTAAAACA | Start: 120 |
| Tm | 58.679 | 59.529 | End: 256 |
| Size | 24 | 20 | 137 |
| Primer 3 details | |||
| Sequence | GGTGTACTGGCAAATTAATGTGA | CGACACGGAGCCACCTAAAA | Start: 121 |
| Tm | 57.363 | 60.32 | End: 258 |
| Size | 23 | 20 | 138 |
| Primer 4 details | |||
| Sequence | AGGTGTACTGGCAAATTAATGTG | CGACACGGAGCCACCTAAAA | Start: 120 |
| Tm | 57.357 | 60.32 | End: 258 |
| Size | 23 | 20 | 139 |
| Primer 5 details | |||
| Sequence | AGGTGTACTGGCAAATTAATGTGAA | CGACACGGAGCCACCTAAAA | Start: 120 |
| Tm | 59.227 | 60.32 | End: 258 |
| Size | 25 | 20 | 139 |
