ssr_primer
Primer Details for TRINITY_DN35531_c0_g1_i1 |
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| (A)13ggtacttcgtactacgttagttgcttgctggtgc(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCCGTTGGACCAGTGTAG | TCTGTAGGAGCAGACGTCGA | Start: 104 |
| Tm | 60.32 | 60.037 | End: 374 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
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| Sequence | CACCCGTTGGACCAGTGTAG | AGGAGCAGACGTCGAAATGG | Start: 104 |
| Tm | 60.32 | 60.109 | End: 369 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
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| Sequence | CTGCCATCTCGTTTGCAGTC | TCTGTAGGAGCAGACGTCGA | Start: 144 |
| Tm | 59.555 | 60.037 | End: 374 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
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| Sequence | CACCCGTTGGACCAGTGTAG | CAGACGTCGAAATGGTTGCG | Start: 104 |
| Tm | 60.32 | 60.179 | End: 364 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
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| Sequence | CTGCCATCTCGTTTGCAGTC | AGGAGCAGACGTCGAAATGG | Start: 144 |
| Tm | 59.555 | 60.109 | End: 369 |
| Size | 20 | 20 | 226 |
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