Primer Details for TRINITY_DN35496_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGAGGAATGTTAGTTGACTAAGA | AGAACATGTGAAGAAAATTGCAAGA | Start: 83 |
| Tm | 57.223 | 58.191 | End: 193 |
| Size | 25 | 25 | 111 |
| Primer 2 details |
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| Sequence | AGTTGACTAAGAATTTGAAAGGTTGCT | AAAGAACATGTGAAGAAAATTGCAAGA | Start: 96 |
| Tm | 59.931 | 59.235 | End: 195 |
| Size | 27 | 27 | 100 |
| Primer 3 details |
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| Sequence | ACTCAAATTTTTGCTTTGAATTTGGT | AGAACATGTGAAGAAAATTGCAAGA | Start: 26 |
| Tm | 57.946 | 58.191 | End: 193 |
| Size | 26 | 25 | 168 |
| Primer 4 details |
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| Sequence | AGTTGACTAAGAATTTGAAAGGTTGCT | AAAGAACATGTGAAGAAAATTGCAA | Start: 96 |
| Tm | 59.931 | 57.153 | End: 195 |
| Size | 27 | 25 | 100 |
| Primer 5 details |
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| Sequence | AGTTGACTAAGAATTTGAAAGGTTGCT | AAAAGAACATGTGAAGAAAATTGCA | Start: 96 |
| Tm | 59.931 | 57.153 | End: 196 |
| Size | 27 | 25 | 101 |
