Primer Details for TRINITY_DN35469_c0_g1_i1 |
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| (AT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACGAACGAAGGGGAGTCG | ACCACAATAGAAATAATGATGTCGGT | Start: 17 |
| Tm | 60.319 | 58.946 | End: 153 |
| Size | 20 | 26 | 137 |
| Primer 2 details |
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| Sequence | CGAAGGGGAGTCGGACTTTT | ACCACAATAGAAATAATGATGTCGGT | Start: 24 |
| Tm | 59.679 | 58.946 | End: 153 |
| Size | 20 | 26 | 130 |
| Primer 3 details |
|---|
| Sequence | AAACGAACGAAGGGGAGTCG | CCACAATAGAAATAATGATGTCGGT | Start: 17 |
| Tm | 60.319 | 57.503 | End: 152 |
| Size | 20 | 25 | 136 |
| Primer 4 details |
|---|
| Sequence | AAACGAACGAAGGGGAGTCG | ACCACAATAGAAATAATGATGTCGG | Start: 17 |
| Tm | 60.319 | 57.503 | End: 153 |
| Size | 20 | 25 | 137 |
| Primer 5 details |
|---|
| Sequence | CGAAGGGGAGTCGGACTTTT | CCACAATAGAAATAATGATGTCGGT | Start: 24 |
| Tm | 59.679 | 57.503 | End: 152 |
| Size | 20 | 25 | 129 |
