Primer Details for TRINITY_DN35273_c0_g1_i1 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTGGTACCCTCCTCAGAGA | TCTACGTTTTGTCTTTAACCAACA | Start: 148 |
| Tm | 58.999 | 57.018 | End: 249 |
| Size | 21 | 24 | 102 |
| Primer 2 details | |||
| Sequence | TCTTGGTACCCTCCTCAGAGAA | TCTACGTTTTGTCTTTAACCAACA | Start: 148 |
| Tm | 59.618 | 57.018 | End: 249 |
| Size | 22 | 24 | 102 |
| Primer 3 details | |||
| Sequence | TTCTTGGTACCCTCCTCAGAGA | TCTACGTTTTGTCTTTAACCAACA | Start: 147 |
| Tm | 59.618 | 57.018 | End: 249 |
| Size | 22 | 24 | 103 |
| Primer 4 details | |||
| Sequence | TCTTGGTACCCTCCTCAGAGA | TCTACGTTTTGTCTTTAACCAACAA | Start: 148 |
| Tm | 58.999 | 57.625 | End: 249 |
| Size | 21 | 25 | 102 |
| Primer 5 details | |||
| Sequence | TCTTGGTACCCTCCTCAGAGA | TTCTACGTTTTGTCTTTAACCAACA | Start: 148 |
| Tm | 58.999 | 57.625 | End: 250 |
| Size | 21 | 25 | 103 |
