Primer Details for TRINITY_DN34935_c0_g1_i1 |
|---|
| (AG)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTCTTCTCAAACGTGGTAGG | GGAACGAGTAGAAGAAACTCATCC | Start: 86 |
| Tm | 59.963 | 58.896 | End: 224 |
| Size | 22 | 24 | 139 |
| Primer 2 details |
|---|
| Sequence | GGTCTTCTCAAACGTGGTAGGT | GGAACGAGTAGAAGAAACTCATCC | Start: 87 |
| Tm | 59.963 | 58.896 | End: 224 |
| Size | 22 | 24 | 138 |
| Primer 3 details |
|---|
| Sequence | TGGTCTTCTCAAACGTGGTAGG | GGAACGAGTAGAAGAAACTCATCCT | Start: 86 |
| Tm | 59.963 | 60.106 | End: 224 |
| Size | 22 | 25 | 139 |
| Primer 4 details |
|---|
| Sequence | GGTCTTCTCAAACGTGGTAGGT | GGAACGAGTAGAAGAAACTCATCCT | Start: 87 |
| Tm | 59.963 | 60.106 | End: 224 |
| Size | 22 | 25 | 138 |
| Primer 5 details |
|---|
| Sequence | TGGTCTTCTCAAACGTGGTAGG | TGGAACGAGTAGAAGAAACTCATCC | Start: 86 |
| Tm | 59.963 | 60.337 | End: 225 |
| Size | 22 | 25 | 140 |
