Primer Details for TRINITY_DN34779_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTACATGTTCTCATTTTGCGT | AGGAACAGAGAAAAGTTGCATGA | Start: 27 |
| Tm | 57.06 | 58.536 | End: 142 |
| Size | 23 | 23 | 116 |
| Primer 2 details | |||
| Sequence | TCTTACATGTTCTCATTTTGCGT | GGAACAGAGAAAAGTTGCATGA | Start: 27 |
| Tm | 57.06 | 57.164 | End: 141 |
| Size | 23 | 22 | 115 |
| Primer 3 details | |||
| Sequence | TCTTACATGTTCTCATTTTGCGT | AGGAACAGAGAAAAGTTGCATG | Start: 27 |
| Tm | 57.06 | 57.157 | End: 142 |
| Size | 23 | 22 | 116 |
| Primer 4 details | |||
| Sequence | TCTTACATGTTCTCATTTTGCGT | AGGAACAGAGAAAAGTTGCATGAA | Start: 27 |
| Tm | 57.06 | 59.111 | End: 142 |
| Size | 23 | 24 | 116 |
| Primer 5 details | |||
| Sequence | ACATGTTCTCATTTTGCGTATACAGT | AGGAACAGAGAAAAGTTGCATGA | Start: 31 |
| Tm | 59.628 | 58.536 | End: 142 |
| Size | 26 | 23 | 112 |
