Primer Details for TRINITY_DN34700_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGTCATAAAAACGAGGGTTCA | TGGCTTAAATATTTCGTTGCGA | Start: 8 |
| Tm | 59.927 | 57.051 | End: 201 |
| Size | 23 | 22 | 194 |
| Primer 2 details | |||
| Sequence | CGAAAGGGTCATAAAAACGAGGG | TGGCTTAAATATTTCGTTGCGA | Start: 4 |
| Tm | 59.874 | 57.051 | End: 201 |
| Size | 23 | 22 | 198 |
| Primer 3 details | |||
| Sequence | AGGGTCATAAAAACGAGGGTTCA | TGGCTTAAATATTTCGTTGCGATGA | Start: 8 |
| Tm | 59.927 | 59.935 | End: 201 |
| Size | 23 | 25 | 194 |
| Primer 4 details | |||
| Sequence | CGAAAGGGTCATAAAAACGAGGG | TGGCTTAAATATTTCGTTGCGATGA | Start: 4 |
| Tm | 59.874 | 59.935 | End: 201 |
| Size | 23 | 25 | 198 |
| Primer 5 details | |||
| Sequence | AGGGTCATAAAAACGAGGGTTCA | TGGCTTAAATATTTCGTTGCGATG | Start: 8 |
| Tm | 59.927 | 58.73 | End: 201 |
| Size | 23 | 24 | 194 |
