Primer Details for TRINITY_DN34299_c0_g1_i1 | |||
|---|---|---|---|
| (T)14: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAGAAGAATGAATGGGCGC | AGAAGGTTACGTCAATGTGTTGG | Start: 20 |
| Tm | 59.934 | 59.187 | End: 119 |
| Size | 21 | 23 | 100 |
| Primer 2 details | |||
| Sequence | GGAAGAAGAATGAATGGGCGC | AAGAAGGTTACGTCAATGTGTTGG | Start: 20 |
| Tm | 59.934 | 59.725 | End: 120 |
| Size | 21 | 24 | 101 |
| Primer 3 details | |||
| Sequence | GGAAGAAGAATGAATGGGCGC | AGAAGGTTACGTCAATGTGTTGGA | Start: 20 |
| Tm | 59.934 | 60.444 | End: 119 |
| Size | 21 | 24 | 100 |
| Primer 4 details | |||
| Sequence | GAAGAAGAATGAATGGGCGCG | AAGAAGGTTACGTCAATGTGTTGG | Start: 21 |
| Tm | 60.268 | 59.725 | End: 120 |
| Size | 21 | 24 | 100 |
| Primer 5 details | |||
| Sequence | AAGAAGAATGAATGGGCGCG | AAAGAAGGTTACGTCAATGTGTTGG | Start: 22 |
| Tm | 59.265 | 60.221 | End: 121 |
| Size | 20 | 25 | 100 |
