ssr_primer
Primer Details for TRINITY_DN3415_c0_g1_i1 |
|---|
| (A)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCAGTGCACCAGACTTAT | AGAACAGGAGCTGCTACAAGT | Start: 131 |
| Tm | 59.678 | 59.026 | End: 272 |
| Size | 20 | 21 | 142 |
| Primer 2 details |
|---|
| Sequence | ACGCAGTGCACCAGACTTAT | GGAGATAGAACAGGAGCTGCT | Start: 131 |
| Tm | 59.678 | 58.961 | End: 278 |
| Size | 20 | 21 | 148 |
| Primer 3 details |
|---|
| Sequence | ACGCAGTGCACCAGACTTAT | AGGAGATAGAACAGGAGCTGC | Start: 131 |
| Tm | 59.678 | 58.961 | End: 279 |
| Size | 20 | 21 | 149 |
| Primer 4 details |
|---|
| Sequence | ACTTGTAGCAGCTCCTGTTCT | GCTGAACATGCCCGAGAGAA | Start: 252 |
| Tm | 59.026 | 60.392 | End: 458 |
| Size | 21 | 20 | 207 |
| Primer 5 details |
|---|
| Sequence | GCAGCTCCTGTTCTATCTCCT | GCTGAACATGCCCGAGAGAA | Start: 259 |
| Tm | 58.961 | 60.392 | End: 458 |
| Size | 21 | 20 | 200 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCAGTGCACCAGACTTAT | AGAACAGGAGCTGCTACAAGT | Start: 131 |
| Tm | 59.678 | 59.026 | End: 272 |
| Size | 20 | 21 | 142 |
| Primer 2 details |
|---|
| Sequence | ACGCAGTGCACCAGACTTAT | GGAGATAGAACAGGAGCTGCT | Start: 131 |
| Tm | 59.678 | 58.961 | End: 278 |
| Size | 20 | 21 | 148 |
| Primer 3 details |
|---|
| Sequence | ACGCAGTGCACCAGACTTAT | AGGAGATAGAACAGGAGCTGC | Start: 131 |
| Tm | 59.678 | 58.961 | End: 279 |
| Size | 20 | 21 | 149 |
| Primer 4 details |
|---|
| Sequence | ACTTGTAGCAGCTCCTGTTCT | GCTGAACATGCCCGAGAGAA | Start: 252 |
| Tm | 59.026 | 60.392 | End: 458 |
| Size | 21 | 20 | 207 |
| Primer 5 details |
|---|
| Sequence | GCAGCTCCTGTTCTATCTCCT | GCTGAACATGCCCGAGAGAA | Start: 259 |
| Tm | 58.961 | 60.392 | End: 458 |
| Size | 21 | 20 | 200 |
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