Primer Details for TRINITY_DN34035_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCACTGAGTGAACTACGCA | ACTTGATCTATTGTTGCTACCCT | Start: 269 |
| Tm | 58.676 | 57.3 | End: 374 |
| Size | 20 | 23 | 106 |
| Primer 2 details | |||
| Sequence | TCCACTGAGTGAACTACGCAA | ACTTGATCTATTGTTGCTACCCT | Start: 269 |
| Tm | 59.316 | 57.3 | End: 374 |
| Size | 21 | 23 | 106 |
| Primer 3 details | |||
| Sequence | TTCCACTGAGTGAACTACGCA | ACTTGATCTATTGTTGCTACCCT | Start: 268 |
| Tm | 59.316 | 57.3 | End: 374 |
| Size | 21 | 23 | 107 |
| Primer 4 details | |||
| Sequence | TCCACTGAGTGAACTACGCA | ACTTGATCTATTGTTGCTACCCTAGT | Start: 269 |
| Tm | 58.676 | 59.616 | End: 374 |
| Size | 20 | 26 | 106 |
| Primer 5 details | |||
| Sequence | AAGAATTTCAATCCTTCCTTTGCA | TGCGTAGTTCACTCAGTGGA | Start: 162 |
| Tm | 57.576 | 58.676 | End: 288 |
| Size | 24 | 20 | 127 |
