ssr_primer
Primer Details for TRINITY_DN33913_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCCTAATTTGGAAGAGGCT | CTCTCAGGAAGCTGTTGGGT | Start: 221 |
| Tm | 59.074 | 59.31 | End: 321 |
| Size | 21 | 20 | 101 |
| Primer 2 details |
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| Sequence | AGCCCTAATTTGGAAGAGGCT | CTCTCAGGAAGCTGTTGGGTT | Start: 221 |
| Tm | 59.074 | 59.927 | End: 321 |
| Size | 21 | 21 | 101 |
| Primer 3 details |
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| Sequence | AGCCCTAATTTGGAAGAGGCTT | CTCTCAGGAAGCTGTTGGGT | Start: 221 |
| Tm | 59.687 | 59.31 | End: 321 |
| Size | 22 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | AGCCCTAATTTGGAAGAGGCT | TCTCAGGAAGCTGTTGGGTT | Start: 221 |
| Tm | 59.074 | 58.86 | End: 320 |
| Size | 21 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | GCCCTAATTTGGAAGAGGCT | CTCTCAGGAAGCTGTTGGGT | Start: 222 |
| Tm | 57.556 | 59.31 | End: 321 |
| Size | 20 | 20 | 100 |
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