ssr_primer
Primer Details for TRINITY_DN3332_c0_g1_i1 |
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| (AAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAGGCGGAGTTGGAGAAG | ATCCACCAATTTCGCCACCT | Start: 224 |
| Tm | 59.965 | 59.96 | End: 429 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
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| Sequence | TTGAGGTTACTTCGGACGGC | ATCCACCAATTTCGCCACCT | Start: 178 |
| Tm | 60.038 | 59.96 | End: 429 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
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| Sequence | CGCCAGAGAGTGTGACGAAT | CTTCTCCAACTCCGCCTTGT | Start: 74 |
| Tm | 60.109 | 59.965 | End: 243 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
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| Sequence | AATCGCCAGAGAGTGTGACG | CTTCTCCAACTCCGCCTTGT | Start: 71 |
| Tm | 60.109 | 59.965 | End: 243 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
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| Sequence | CGCCAGAGAGTGTGACGAAT | GCCGTCCGAAGTAACCTCAA | Start: 74 |
| Tm | 60.109 | 60.038 | End: 197 |
| Size | 20 | 20 | 124 |
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