ssr_primer
Primer Details for TRINITY_DN33282_c0_g1_i1 |
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| (GAAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGATCGAGAACCGCTCATCA | TGATTGCTCCTTGGCGGATT | Start: 17 |
| Tm | 59.971 | 60.034 | End: 128 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
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| Sequence | CGATCGAGAACCGCTCATCA | ATCACTGCCGAGGAATTGCA | Start: 17 |
| Tm | 59.971 | 60.035 | End: 172 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
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| Sequence | TGCAATTCCTCGGCAGTGAT | TCTGTTCCTCTGAGCTCGGA | Start: 153 |
| Tm | 60.035 | 59.962 | End: 390 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
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| Sequence | ATCAATGTAGCCATCGCCGT | TCGCTGAACTCGAGCAAGTT | Start: 278 |
| Tm | 59.893 | 59.968 | End: 444 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
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| Sequence | CTGAAGCTCTTCCTCGGTGG | TCGCTGAACTCGAGCAAGTT | Start: 323 |
| Tm | 60.108 | 59.968 | End: 444 |
| Size | 20 | 20 | 122 |
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