Primer Details for TRINITY_DN33087_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTATGCCACACTCTCACC | TGGTTTCCTATCTTCTGTTCCTTGA | Start: 79 |
| Tm | 59.469 | 59.69 | End: 182 |
| Size | 20 | 25 | 104 |
| Primer 2 details |
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| Sequence | CGTATGCCACACTCTCACCT | TGGTTTCCTATCTTCTGTTCCTTGA | Start: 80 |
| Tm | 59.467 | 59.69 | End: 182 |
| Size | 20 | 25 | 103 |
| Primer 3 details |
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| Sequence | TCGTATGCCACACTCTCACC | TGATGGTTTCCTATCTTCTGTTCCT | Start: 79 |
| Tm | 59.469 | 59.511 | End: 185 |
| Size | 20 | 25 | 107 |
| Primer 4 details |
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| Sequence | CGTATGCCACACTCTCACCT | TGATGGTTTCCTATCTTCTGTTCCT | Start: 80 |
| Tm | 59.467 | 59.511 | End: 185 |
| Size | 20 | 25 | 106 |
| Primer 5 details |
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| Sequence | TCGTATGCCACACTCTCACC | TGGTTTCCTATCTTCTGTTCCTTG | Start: 79 |
| Tm | 59.469 | 58.439 | End: 182 |
| Size | 20 | 24 | 104 |
