Primer Details for TRINITY_DN33062_c0_g1_i1 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTCAACACGTAGAAATTAACCT | TGGCAGTGTTGTGTGACATTG | Start: 78 |
| Tm | 58.138 | 59.595 | End: 270 |
| Size | 24 | 21 | 193 |
| Primer 2 details | |||
| Sequence | ACCTCAACACGTAGAAATTAACCT | GGCAGTGTTGTGTGACATTGA | Start: 78 |
| Tm | 58.138 | 59.325 | End: 269 |
| Size | 24 | 21 | 192 |
| Primer 3 details | |||
| Sequence | ACCTCAACACGTAGAAATTAACCT | TGGCAGTGTTGTGTGACATT | Start: 78 |
| Tm | 58.138 | 58.239 | End: 270 |
| Size | 24 | 20 | 193 |
| Primer 4 details | |||
| Sequence | ACCTCAACACGTAGAAATTAACCTT | TGGCAGTGTTGTGTGACATTG | Start: 78 |
| Tm | 58.706 | 59.595 | End: 270 |
| Size | 25 | 21 | 193 |
| Primer 5 details | |||
| Sequence | ACCTCAACACGTAGAAATTAACCT | ATGGCAGTGTTGTGTGACAT | Start: 78 |
| Tm | 58.138 | 58.015 | End: 271 |
| Size | 24 | 20 | 194 |
