Primer Details for TRINITY_DN32934_c0_g1_i1 |
|---|
| (AT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAAAGAAATTGTTCCTTTTACCCA | ACATTTTCAGATAAACAACAGATGGA | Start: 118 |
| Tm | 59.459 | 57.592 | End: 218 |
| Size | 26 | 26 | 101 |
| Primer 2 details |
|---|
| Sequence | AGCAAAAGAAATTGTTCCTTTTACCC | ACATTTTCAGATAAACAACAGATGGA | Start: 117 |
| Tm | 59.234 | 57.592 | End: 218 |
| Size | 26 | 26 | 102 |
| Primer 3 details |
|---|
| Sequence | GCAAAAGAAATTGTTCCTTTTACCC | ACATTTTCAGATAAACAACAGATGGA | Start: 118 |
| Tm | 58.045 | 57.592 | End: 218 |
| Size | 25 | 26 | 101 |
| Primer 4 details |
|---|
| Sequence | GCAAAAGAAATTGTTCCTTTTACCCA | TGCTAATTGAGTTGTAGTTCCATAAA | Start: 118 |
| Tm | 59.459 | 57.144 | End: 301 |
| Size | 26 | 26 | 184 |
| Primer 5 details |
|---|
| Sequence | GCAAAAGAAATTGTTCCTTTTACCCA | ACATTTTCAGATAAACAACAGATGGAA | Start: 118 |
| Tm | 59.459 | 58.141 | End: 218 |
| Size | 26 | 27 | 101 |
