Primer Details for TRINITY_DN32855_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAATTTTGTAGCATTTAAGACGA | TCTTTCCCTTTTCCAAATTGCCA | Start: 41 |
| Tm | 58.327 | 59.286 | End: 141 |
| Size | 25 | 23 | 101 |
| Primer 2 details |
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| Sequence | TGCAATTTTGTAGCATTTAAGACG | TCTTTCCCTTTTCCAAATTGCCA | Start: 41 |
| Tm | 57.074 | 59.286 | End: 141 |
| Size | 24 | 23 | 101 |
| Primer 3 details |
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| Sequence | TGCAATTTTGTAGCATTTAAGACGA | CTTTCCCTTTTCCAAATTGCCA | Start: 41 |
| Tm | 58.327 | 57.916 | End: 140 |
| Size | 25 | 22 | 100 |
| Primer 4 details |
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| Sequence | TGCAATTTTGTAGCATTTAAGACGA | TCTTTCCCTTTTCCAAATTGCCAA | Start: 41 |
| Tm | 58.327 | 59.835 | End: 141 |
| Size | 25 | 24 | 101 |
| Primer 5 details |
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| Sequence | TGCAATTTTGTAGCATTTAAGACGAA | TCTTTCCCTTTTCCAAATTGCCA | Start: 41 |
| Tm | 58.858 | 59.286 | End: 141 |
| Size | 26 | 23 | 101 |
