Primer Details for TRINITY_DN3271_c0_g1_i1 | |||
|---|---|---|---|
| (T)17: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGAACCTCTTAAAACCTTTGCA | CCCAGCAGATGTGACTTTTCC | Start: 127 |
| Tm | 59.046 | 59.185 | End: 235 |
| Size | 24 | 21 | 109 |
| Primer 2 details | |||
| Sequence | TCAGTGAACCTCTTAAAACCTTTGC | CCCAGCAGATGTGACTTTTCC | Start: 125 |
| Tm | 59.933 | 59.185 | End: 235 |
| Size | 25 | 21 | 111 |
| Primer 3 details | |||
| Sequence | CAGTGAACCTCTTAAAACCTTTGCA | CCCAGCAGATGTGACTTTTCC | Start: 126 |
| Tm | 60.164 | 59.185 | End: 235 |
| Size | 25 | 21 | 110 |
| Primer 4 details | |||
| Sequence | GTGAACCTCTTAAAACCTTTGCA | CCCAGCAGATGTGACTTTTCC | Start: 128 |
| Tm | 57.743 | 59.185 | End: 235 |
| Size | 23 | 21 | 108 |
| Primer 5 details | |||
| Sequence | CAGTGAACCTCTTAAAACCTTTGC | CCCAGCAGATGTGACTTTTCC | Start: 126 |
| Tm | 58.718 | 59.185 | End: 235 |
| Size | 24 | 21 | 110 |
