Primer Details for TRINITY_DN32282_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCACTCACCATTAAGGCA | ACACACATTTTAGGAGTGAAGTGA | Start: 64 |
| Tm | 58.633 | 58.319 | End: 230 |
| Size | 20 | 24 | 167 |
| Primer 2 details | |||
| Sequence | TCCCACTCACCATTAAGGCAA | ACACACATTTTAGGAGTGAAGTGA | Start: 64 |
| Tm | 59.292 | 58.319 | End: 230 |
| Size | 21 | 24 | 167 |
| Primer 3 details | |||
| Sequence | TTCCCACTCACCATTAAGGCA | ACACACATTTTAGGAGTGAAGTGA | Start: 63 |
| Tm | 59.292 | 58.319 | End: 230 |
| Size | 21 | 24 | 168 |
| Primer 4 details | |||
| Sequence | TCCCACTCACCATTAAGGCA | ACACACATTTTAGGAGTGAAGTGAA | Start: 64 |
| Tm | 58.633 | 58.88 | End: 230 |
| Size | 20 | 25 | 167 |
| Primer 5 details | |||
| Sequence | TCCCACTCACCATTAAGGCA | AACACACATTTTAGGAGTGAAGTGA | Start: 64 |
| Tm | 58.633 | 58.88 | End: 231 |
| Size | 20 | 25 | 168 |
