Primer Details for TRINITY_DN32110_c0_g1_i1 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTAGGATAATTAGCTTGAGCCA | GGAGTAGGTAGTGGCAGAGC | Start: 16 |
| Tm | 59.633 | 59.252 | End: 174 |
| Size | 25 | 20 | 159 |
| Primer 2 details |
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| Sequence | ACACTAGGATAATTAGCTTGAGCCA | CAGAAATTGGCCAGGTTGAGC | Start: 16 |
| Tm | 59.633 | 60.067 | End: 154 |
| Size | 25 | 21 | 139 |
| Primer 3 details |
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| Sequence | ACACTAGGATAATTAGCTTGAGCCA | AGAAATTGGCCAGGTTGAGCT | Start: 16 |
| Tm | 59.633 | 60.203 | End: 153 |
| Size | 25 | 21 | 138 |
| Primer 4 details |
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| Sequence | CACTAGGATAATTAGCTTGAGCCA | GGAGTAGGTAGTGGCAGAGC | Start: 17 |
| Tm | 58.142 | 59.252 | End: 174 |
| Size | 24 | 20 | 158 |
| Primer 5 details |
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| Sequence | ACACTAGGATAATTAGCTTGAGCC | GGAGTAGGTAGTGGCAGAGC | Start: 16 |
| Tm | 58.141 | 59.252 | End: 174 |
| Size | 24 | 20 | 159 |
