Primer Details for TRINITY_DN32099_c0_g1_i1 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTCGAGACATACAAAAGACTACA | TTCCAATCCGCTTGCGTTTC | Start: 212 |
| Tm | 58.259 | 59.76 | End: 373 |
| Size | 25 | 20 | 162 |
| Primer 2 details |
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| Sequence | TGTTCGAGACATACAAAAGACTACA | CTTCCAATCCGCTTGCGTTT | Start: 212 |
| Tm | 58.259 | 59.759 | End: 374 |
| Size | 25 | 20 | 163 |
| Primer 3 details |
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| Sequence | TGTTCGAGACATACAAAAGACTACA | TCCAATCCGCTTGCGTTTCT | Start: 212 |
| Tm | 58.259 | 60.605 | End: 372 |
| Size | 25 | 20 | 161 |
| Primer 4 details |
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| Sequence | TGTTCGAGACATACAAAAGACTACAA | TTCCAATCCGCTTGCGTTTC | Start: 212 |
| Tm | 58.795 | 59.76 | End: 373 |
| Size | 26 | 20 | 162 |
| Primer 5 details |
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| Sequence | TGTTCGAGACATACAAAAGACTACAA | CTTCCAATCCGCTTGCGTTT | Start: 212 |
| Tm | 58.795 | 59.759 | End: 374 |
| Size | 26 | 20 | 163 |
