Primer Details for TRINITY_DN32058_c0_g1_i1 | |||
|---|---|---|---|
| (A)16: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTCATCAGCATTCATGTCATGT | AGTCTCTCATGCAAAAAGGGGA | Start: 53 |
| Tm | 59.297 | 59.623 | End: 230 |
| Size | 24 | 22 | 178 |
| Primer 2 details | |||
| Sequence | TGTTCATCAGCATTCATGTCATGT | GTCTCTCATGCAAAAAGGGGA | Start: 53 |
| Tm | 59.297 | 58.205 | End: 229 |
| Size | 24 | 21 | 177 |
| Primer 3 details | |||
| Sequence | TGTTCATCAGCATTCATGTCATGT | AGTCTCTCATGCAAAAAGGGG | Start: 53 |
| Tm | 59.297 | 58.2 | End: 230 |
| Size | 24 | 21 | 178 |
| Primer 4 details | |||
| Sequence | TGTTCATCAGCATTCATGTCATGTT | AGTCTCTCATGCAAAAAGGGGA | Start: 53 |
| Tm | 59.816 | 59.623 | End: 230 |
| Size | 25 | 22 | 178 |
| Primer 5 details | |||
| Sequence | TGTTCATCAGCATTCATGTCATG | AGTCTCTCATGCAAAAAGGGGA | Start: 53 |
| Tm | 57.757 | 59.623 | End: 230 |
| Size | 23 | 22 | 178 |
