Primer Details for TRINITY_DN31976_c0_g1_i1 | |||
|---|---|---|---|
| (A)15: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCGCCTTAACAACACTGC | TCAAACTACTACAAAGTGCAAACT | Start: 7 |
| Tm | 59.97 | 57.004 | End: 151 |
| Size | 20 | 24 | 145 |
| Primer 2 details | |||
| Sequence | GCGCCTTAACAACACTGCAT | TCAAACTACTACAAAGTGCAAACT | Start: 9 |
| Tm | 59.759 | 57.004 | End: 151 |
| Size | 20 | 24 | 143 |
| Primer 3 details | |||
| Sequence | TTGCGCCTTAACAACACTGC | ATCAAACTACTACAAAGTGCAAACT | Start: 7 |
| Tm | 59.97 | 57.432 | End: 152 |
| Size | 20 | 25 | 146 |
| Primer 4 details | |||
| Sequence | CGGTTGCGCCTTAACAACAC | TCAAACTACTACAAAGTGCAAACT | Start: 4 |
| Tm | 60.659 | 57.004 | End: 151 |
| Size | 20 | 24 | 148 |
| Primer 5 details | |||
| Sequence | GGTTGCGCCTTAACAACACT | TCAAACTACTACAAAGTGCAAACT | Start: 5 |
| Tm | 59.334 | 57.004 | End: 151 |
| Size | 20 | 24 | 147 |
