Primer Details for TRINITY_DN31748_c0_g1_i1 | |||
|---|---|---|---|
| (T)14: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGATCTTTGCATTTCTTGTTGA | ACTTCCTTTCCAGTTTCCACCA | Start: 49 |
| Tm | 57.563 | 59.756 | End: 185 |
| Size | 23 | 22 | 137 |
| Primer 2 details | |||
| Sequence | TCATTTGACTTGTACGATCTTTGCA | ACTTCCTTTCCAGTTTCCACCA | Start: 36 |
| Tm | 59.532 | 59.756 | End: 185 |
| Size | 25 | 22 | 150 |
| Primer 3 details | |||
| Sequence | TGCATTTCTTGTTGAACATCCTT | ACTTCCTTTCCAGTTTCCACCA | Start: 57 |
| Tm | 57.466 | 59.756 | End: 185 |
| Size | 23 | 22 | 129 |
| Primer 4 details | |||
| Sequence | TTGCATTTCTTGTTGAACATCCT | ACTTCCTTTCCAGTTTCCACCA | Start: 56 |
| Tm | 57.466 | 59.756 | End: 185 |
| Size | 23 | 22 | 130 |
| Primer 5 details | |||
| Sequence | CGATCTTTGCATTTCTTGTTGAACA | ACTTCCTTTCCAGTTTCCACCA | Start: 50 |
| Tm | 59.313 | 59.756 | End: 185 |
| Size | 25 | 22 | 136 |
