Primer Details for TRINITY_DN31588_c0_g1_i1 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGGAGACATACTTCTCTTTATCCCA | TGGAAGGTCGGTTTTGCCTT | Start: 33 |
| Tm | 59.675 | 60.106 | End: 156 |
| Size | 26 | 20 | 124 |
| Primer 2 details |
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| Sequence | ACAGGAGACATACTTCTCTTTATCCC | TGGAAGGTCGGTTTTGCCTT | Start: 32 |
| Tm | 59.675 | 60.106 | End: 156 |
| Size | 26 | 20 | 125 |
| Primer 3 details |
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| Sequence | CAGGAGACATACTTCTCTTTATCCCA | ATGGAAGGTCGGTTTTGCCT | Start: 33 |
| Tm | 59.675 | 59.888 | End: 157 |
| Size | 26 | 20 | 125 |
| Primer 4 details |
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| Sequence | ACAGGAGACATACTTCTCTTTATCCC | ATGGAAGGTCGGTTTTGCCT | Start: 32 |
| Tm | 59.675 | 59.888 | End: 157 |
| Size | 26 | 20 | 126 |
| Primer 5 details |
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| Sequence | AGGAGACATACTTCTCTTTATCCCA | TGGAAGGTCGGTTTTGCCTT | Start: 34 |
| Tm | 58.555 | 60.106 | End: 156 |
| Size | 25 | 20 | 123 |
