Primer Details for TRINITY_DN31583_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGTTGTAAGAATAACTTCGATGG | AGGTCAAAATCTTAAAGTTAGGAACA | Start: 17 |
| Tm | 59.42 | 57.01 | End: 138 |
| Size | 25 | 26 | 122 |
| Primer 2 details |
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| Sequence | ACCGTTGTAAGAATAACTTCGATGG | GAGGTCAAAATCTTAAAGTTAGGAACA | Start: 17 |
| Tm | 59.42 | 57.936 | End: 139 |
| Size | 25 | 27 | 123 |
| Primer 3 details |
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| Sequence | CCGTTGTAAGAATAACTTCGATGGA | AGGTCAAAATCTTAAAGTTAGGAACA | Start: 18 |
| Tm | 59.191 | 57.01 | End: 138 |
| Size | 25 | 26 | 121 |
| Primer 4 details |
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| Sequence | GTGAACTCTCCATAATAACCGTTGT | AGGTCAAAATCTTAAAGTTAGGAACA | Start: 0 |
| Tm | 59.127 | 57.01 | End: 138 |
| Size | 25 | 26 | 139 |
| Primer 5 details |
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| Sequence | ACCGTTGTAAGAATAACTTCGATGG | AGAGGTCAAAATCTTAAAGTTAGGAAC | Start: 17 |
| Tm | 59.42 | 57.714 | End: 140 |
| Size | 25 | 27 | 124 |
