ssr_primer
Primer Details for TRINITY_DN31527_c0_g1_i1 |
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| (A)19gttccccttggcgataaaagacttaatctcaaagatttggcatggatacttaatcttctgtt(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCCTTGGCGATAAAAGACT | AGAAGTCATGTCTGGCAACCA | Start: 114 |
| Tm | 59.087 | 59.579 | End: 235 |
| Size | 21 | 21 | 122 |
| Primer 2 details |
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| Sequence | AAAAAGTTCCCCTTGGCGAT | AGAAGTCATGTCTGGCAACCA | Start: 107 |
| Tm | 57.99 | 59.579 | End: 235 |
| Size | 20 | 21 | 129 |
| Primer 3 details |
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| Sequence | TGGTTGCCAGACATGACTTCT | AGAGTCCCATTGTTTGCCTTCA | Start: 215 |
| Tm | 59.579 | 60.158 | End: 419 |
| Size | 21 | 22 | 205 |
| Primer 4 details |
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| Sequence | TGGTTGCCAGACATGACTTCT | GAGTCCCATTGTTTGCCTTCA | Start: 215 |
| Tm | 59.579 | 58.761 | End: 418 |
| Size | 21 | 21 | 204 |
| Primer 5 details |
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| Sequence | TCCCCTTGGCGATAAAAGACTT | AGAAGTCATGTCTGGCAACCA | Start: 114 |
| Tm | 59.692 | 59.579 | End: 235 |
| Size | 22 | 21 | 122 |
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