Primer Details for TRINITY_DN31441_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGGTTTGTTCGGTGTTGT | ACAATACTACAATCCTGATTCATGTTT | Start: 59 |
| Tm | 60.393 | 57.534 | End: 185 |
| Size | 20 | 27 | 127 |
| Primer 2 details |
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| Sequence | TCGGGTTTGTTCGGTGTTGT | AACAATACTACAATCCTGATTCATGTT | Start: 59 |
| Tm | 60.393 | 57.534 | End: 186 |
| Size | 20 | 27 | 128 |
| Primer 3 details |
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| Sequence | TCGGGTTTGTTCGGTGTTGT | AAACAATACTACAATCCTGATTCATGT | Start: 59 |
| Tm | 60.393 | 57.534 | End: 187 |
| Size | 20 | 27 | 129 |
| Primer 4 details |
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| Sequence | TCGGGTTTGTTCGGTGTTGT | TTCAAACAATACTACAATCCTGATTCA | Start: 59 |
| Tm | 60.393 | 57.486 | End: 190 |
| Size | 20 | 27 | 132 |
| Primer 5 details |
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| Sequence | TCGGGTTTGTTCGGTGTTGT | TCAAACAATACTACAATCCTGATTCAT | Start: 59 |
| Tm | 60.393 | 57.315 | End: 189 |
| Size | 20 | 27 | 131 |
