Primer Details for TRINITY_DN31412_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTATAGGCCAGGTGGAGA | GCTTCCTTTGTTGTTGAAAATGACG | Start: 99 |
| Tm | 59.058 | 60.276 | End: 315 |
| Size | 20 | 25 | 217 |
| Primer 2 details | |||
| Sequence | ACCTATAGGCCAGGTGGAGA | TCCTTTGTTGTTGAAAATGACGT | Start: 99 |
| Tm | 59.058 | 57.682 | End: 312 |
| Size | 20 | 23 | 214 |
| Primer 3 details | |||
| Sequence | ACCTATAGGCCAGGTGGAGA | AGCATTGTGTACAATCAAAACCA | Start: 99 |
| Tm | 59.058 | 57.472 | End: 279 |
| Size | 20 | 23 | 181 |
| Primer 4 details | |||
| Sequence | ACCTATAGGCCAGGTGGAGA | TCCTTTGTTGTTGAAAATGACGTT | Start: 99 |
| Tm | 59.058 | 58.285 | End: 312 |
| Size | 20 | 24 | 214 |
| Primer 5 details | |||
| Sequence | ACCTATAGGCCAGGTGGAGA | TTCCTTTGTTGTTGAAAATGACGT | Start: 99 |
| Tm | 59.058 | 58.285 | End: 313 |
| Size | 20 | 24 | 215 | (A)22: Primer 1 details |
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTATAGGCCAGGTGGAGA | GCTTCCTTTGTTGTTGAAAATGACG | Start: 99 |
| Tm | 59.058 | 60.276 | End: 315 |
| Size | 20 | 25 | 217 |
| Primer 2 details | |||
| Sequence | ACCTATAGGCCAGGTGGAGA | TCCTTTGTTGTTGAAAATGACGT | Start: 99 |
| Tm | 59.058 | 57.682 | End: 312 |
| Size | 20 | 23 | 214 |
| Primer 3 details | |||
| Sequence | ACCTATAGGCCAGGTGGAGA | AGCATTGTGTACAATCAAAACCA | Start: 99 |
| Tm | 59.058 | 57.472 | End: 279 |
| Size | 20 | 23 | 181 |
| Primer 4 details | |||
| Sequence | ACCTATAGGCCAGGTGGAGA | TCCTTTGTTGTTGAAAATGACGTT | Start: 99 |
| Tm | 59.058 | 58.285 | End: 312 |
| Size | 20 | 24 | 214 |
| Primer 5 details | |||
| Sequence | ACCTATAGGCCAGGTGGAGA | TTCCTTTGTTGTTGAAAATGACGT | Start: 99 |
| Tm | 59.058 | 58.285 | End: 313 |
| Size | 20 | 24 | 215 |
