Primer Details for TRINITY_DN31374_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAGAAGGTGGCTGTGGAT | AGAGATTTGAAGACTTTGAACCTCT | Start: 45 |
| Tm | 60.549 | 58.164 | End: 180 |
| Size | 20 | 25 | 136 |
| Primer 2 details |
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| Sequence | AGGAGAAGGTGGCTGTGGAT | AGAGATTTGAAGACTTTGAACCTCTT | Start: 45 |
| Tm | 60.549 | 58.714 | End: 180 |
| Size | 20 | 26 | 136 |
| Primer 3 details |
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| Sequence | GGTGGCTGTGGATATCCTCA | AGAGATTTGAAGACTTTGAACCTCT | Start: 52 |
| Tm | 58.868 | 58.164 | End: 180 |
| Size | 20 | 25 | 129 |
| Primer 4 details |
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| Sequence | AGGAGAAGGTGGCTGTGGA | AGAGATTTGAAGACTTTGAACCTCT | Start: 45 |
| Tm | 60.152 | 58.164 | End: 180 |
| Size | 19 | 25 | 136 |
| Primer 5 details |
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| Sequence | GAGGAGAAGGTGGCTGTGGA | AGAGATTTGAAGACTTTGAACCTCT | Start: 44 |
| Tm | 61.195 | 58.164 | End: 180 |
| Size | 20 | 25 | 137 |
