Primer Details for TRINITY_DN31184_c0_g1_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACATTGTGTATGGGGCTACA | TGCTTTTTGGACATACTACAATGGT | Start: 77 |
| Tm | 59.426 | 59.227 | End: 181 |
| Size | 22 | 25 | 105 |
| Primer 2 details |
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| Sequence | ACTTTCACATTGTGTATGGGGC | TGCTTTTTGGACATACTACAATGGT | Start: 73 |
| Tm | 59.174 | 59.227 | End: 181 |
| Size | 22 | 25 | 109 |
| Primer 3 details |
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| Sequence | TTCACATTGTGTATGGGGCT | TGCTTTTTGGACATACTACAATGGT | Start: 76 |
| Tm | 57.103 | 59.227 | End: 181 |
| Size | 20 | 25 | 106 |
| Primer 4 details |
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| Sequence | CACATTGTGTATGGGGCTACA | TGCTTTTTGGACATACTACAATGGT | Start: 78 |
| Tm | 58.002 | 59.227 | End: 181 |
| Size | 21 | 25 | 104 |
| Primer 5 details |
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| Sequence | TTCACATTGTGTATGGGGCTACA | TGCTTTTTGGACATACTACAATGGT | Start: 76 |
| Tm | 59.99 | 59.227 | End: 181 |
| Size | 23 | 25 | 106 |
