Primer Details for TRINITY_DN31031_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCTGAATTTAAGATTTGCCAA | TGGGGTATTGGAGAATCATTAAAGTG | Start: 38 |
| Tm | 57.256 | 59.167 | End: 314 |
| Size | 23 | 26 | 277 |
| Primer 2 details |
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| Sequence | AAGGCTGAATTTAAGATTTGCCA | TGGGGTATTGGAGAATCATTAAAGTG | Start: 37 |
| Tm | 57.256 | 59.167 | End: 314 |
| Size | 23 | 26 | 278 |
| Primer 3 details |
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| Sequence | AGGCTGAATTTAAGATTTGCCAA | TGTCTTTTGGTTTCGTTAGATTCA | Start: 38 |
| Tm | 57.256 | 57.071 | End: 143 |
| Size | 23 | 24 | 106 |
| Primer 4 details |
|---|
| Sequence | AAGGCTGAATTTAAGATTTGCCA | TGTCTTTTGGTTTCGTTAGATTCA | Start: 37 |
| Tm | 57.256 | 57.071 | End: 143 |
| Size | 23 | 24 | 107 |
| Primer 5 details |
|---|
| Sequence | AGGCTGAATTTAAGATTTGCCAA | TGGGGTATTGGAGAATCATTAAAGT | Start: 38 |
| Tm | 57.256 | 58.019 | End: 314 |
| Size | 23 | 25 | 277 |
