Primer Details for TRINITY_DN30869_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAATTCTCCTTAAAACAATGTGCT | ACTCATAACCCTCATTAATCACTTCA | Start: 58 |
| Tm | 57.946 | 58.138 | End: 191 |
| Size | 25 | 26 | 134 |
| Primer 2 details |
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| Sequence | ACAATTCTCCTTAAAACAATGTGCT | TGAACTCATAACCCTCATTAATCACT | Start: 58 |
| Tm | 57.946 | 58.138 | End: 194 |
| Size | 25 | 26 | 137 |
| Primer 3 details |
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| Sequence | AACAATTCTCCTTAAAACAATGTGCT | ACTCATAACCCTCATTAATCACTTCA | Start: 57 |
| Tm | 58.5 | 58.138 | End: 191 |
| Size | 26 | 26 | 135 |
| Primer 4 details |
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| Sequence | AACAATTCTCCTTAAAACAATGTGCT | TGAACTCATAACCCTCATTAATCACT | Start: 57 |
| Tm | 58.5 | 58.138 | End: 194 |
| Size | 26 | 26 | 138 |
| Primer 5 details |
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| Sequence | ACAATTCTCCTTAAAACAATGTGCT | AACTCATAACCCTCATTAATCACTTCA | Start: 58 |
| Tm | 57.946 | 58.671 | End: 192 |
| Size | 25 | 27 | 135 |
